Question 1

How do I reconstitute a peptide?

Accepted Answer

To reconstitute a peptide for in-vitro research, inject bacteriostatic water slowly down the inside wall of the vial containing lyophilized peptide powder. Allow the water to dissolve the powder gently — do not shake or agitate. The resulting solution concentration depends on the amount of water added relative to the peptide mass. Use our free reconstitution calculator to determine exact volumes for your research protocol.

Question 2

How much bacteriostatic water should I use?

Accepted Answer

The amount of bacteriostatic water depends on the peptide mass and your desired concentration for laboratory research. Common reconstitution volumes range from 1 mL to 3 mL per vial. Using more water creates a more dilute solution with easier-to-measure doses, while less water creates a more concentrated solution requiring smaller injection volumes. Our reconstitution calculator automatically determines the optimal water volume based on your peptide and target dose.

Question 3

How do I calculate peptide dosage in units?

Accepted Answer

To calculate peptide dosage in insulin syringe units for research use, divide your desired dose (in mcg) by the concentration per unit. The concentration per unit depends on how much peptide was reconstituted and with how much water. For example, 5 mg of peptide in 2 mL of water yields 2,500 mcg/mL or 25 mcg per unit on a 100-unit syringe. Our dosage calculator performs this math automatically and shows exact syringe markings.

Question 4

How long do reconstituted peptides last?

Accepted Answer

Reconstituted peptides mixed with bacteriostatic water typically remain stable for 28-30 days when stored at 2-8°C (standard refrigerator temperature) in a laboratory setting. Peptides mixed with sterile water without preservatives should be used within 24-48 hours. Stability varies by peptide — some like BPC-157 are more robust, while others like certain growth hormone releasing peptides degrade faster. Always store reconstituted vials upright and away from light.

Question 5

What syringe size should I use for peptides?

Accepted Answer

For peptide research, insulin syringes are the standard choice due to their fine graduations and precision. A 1 mL (100-unit) insulin syringe is most common and works well for doses above 10 units. For smaller, more precise doses, a 0.5 mL (50-unit) or 0.3 mL (30-unit) syringe provides finer graduation marks. The syringe gauge (needle thickness) is typically 29-31 gauge. Our unit converter tool helps translate your calculated dose into the correct syringe markings.

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Lyophilized (Powder)

Reconstituted

During Use

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